DNA Library

(Construction of DNA Saturation Mutagenesis Library)

Controlled randomization mutagenesis technique can be identified as a new technique in protein engineering, by which the targeted amino acid can be replaced by any one of the other 19 amino acids with random substitution. This technique is not only a powerful tool for directed evolution of a certain protein with improved or novel properties but also, it is an important method to research the protein structure and function relationship. We can process saturation mutagenesis in the position associated with certain function of protein appointed by custom to get a required DNA library.

Applications:

• Improvement of activity of proteins
• Improvement of industrial enzymes
• Affinity maturation of antibodies
• Physicochemical improvement (e.g. dissolubility, heat stabilization, ligase combination specialty)
• Agriculture science, petrol chemistry, bioengineering

Highlights of Our DNA Library Service

• Diversities of up to 1014 achievable
• highly directed and specialty
• Ultra fast delivery times (as fast as ~4 weeks)
• Low ancillary mutation rate
• Less silent mutations

Quality control:

• Bulk sequencing of amplified and cloned library;
• Individual clone analysis(depends on your requirement).

The statistical analysis of the final library includes:

• Flanking sequence analysis;
• Even distribution of nucleotides at the degenerated sites;
• RT-PCT to verify the number of distinct molecules in the non-amplified library if necessary.

Supplies and Deliverables

Customer To Supply:

• Sequence of amino acid with mutagenesis position or gene sequence with template with expected mutagenesis position;
• The name of clone vector and clone point;
• The Latin name of host cell.

Biomatik To Deliver:

Item	Specs
DNA library before clone	5µg DNA（with 5' and 3' restriction sites）in line
DNA library after clone	10µg DNA (customer's vector) in plasmid, Glycerol Stock

For DNA Library Order or Enquiry

• Email: custom@biomatik.com
• Phone: 1-800-836-8089(USA); 1-519-489-7195(International)
• Fax: 1-877-221-3515(USA); 1-519-231-0140(International)

* Download Purchase Order Form if you don't have one.