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Human Lactate Dehydrogenase A (LDHA) ELISA Kit

Human Lactate Dehydrogenase A (LDHA) ELISA Kit

Cat. No. EKU05532
Product Name Human Lactate Dehydrogenase A (LDHA) ELISA Kit
Estimated Turnaround Time 7-11 business days

Product Description

Species Reactivity Homo sapiens (Human)
Format 96 well plate
Detection Range 0.156-10 ng/mL
Sensitivity 0.067 ng/mL
Sample Type Serum, plasma, tissue homogenates and other biological fluids
Assay Time 3 hours
UniProt P00338
Gene ID 3939
Gene Name Lactate Dehydrogenase A
Gene Aliases LDH-A; LDH-M; LDH1; PIG19; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; Renal carcinoma antigen NY-REN-59
Application ELISA
Assay Type Sandwich
Detection Method Colorimetric
Shipping Condition Blue ice
Storage Instructions -20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date 8 months
Specificity This assay has high sensitivity and excellent specificity for detection of Lactate Dehydrogenase A (LDHA). No significant cross-reactivity or interference between Lactate Dehydrogenase A (LDHA) and analogues was observed.
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactate Dehydrogenase A (LDHA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactate Dehydrogenase A (LDHA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactate Dehydrogenase A (LDHA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lactate Dehydrogenase A (LDHA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactate Dehydrogenase A (LDHA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and
Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions For research use only. Not for diagnostic procedures.
Quality Systems The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area Enzyme & Kinase;Metabolic pathway;Cardiovascular biology;Hepatology;
Important Notes The above product specifications may be updated as a result of continuous improvements.
Please refer to hard copy manual included in the kit for your experiment.

 

Cat. No. Name Size Price (USD) Qty Add to Cart
EKU05532 Human Lactate Dehydrogenase A (LDHA) ELISA Kit 96T $698.00
EKU05532 Human Lactate Dehydrogenase A (LDHA) ELISA Kit 5x96T $3,108.00
EKU05532 Human Lactate Dehydrogenase A (LDHA) ELISA Kit 10x96T $5,657.00

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