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Human Mothers Against Decapentaplegic Homolog 9 (Smad9) ELISA Kit

Human Mothers Against Decapentaplegic Homolog 9 (Smad9) ELISA Kit

Cat. No. EKU10157
Product Name Human Mothers Against Decapentaplegic Homolog 9 (Smad9) ELISA Kit
Species Reactivity Homo sapiens (Human)
Estimated Turnaround Time 6-11 Business days

Product Description

Format 96-Well Plate
Target Name Mothers Against Decapentaplegic Homolog 9
Target Synonyms MADH2, MADR2, Madh6, Mothers against DPP homolog 9
Sample Type Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range 0.156-10 ng/mL
Sensitivity 0.061 ng/mL
Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 9 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 9 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV<10%;Inter-Assay: CV<12%
Detection Method Colorimetric
Assay Time 3 hours
Application ELISA
Assay Type Double-antibody Sandwich
Shipping Condition Ice packs
UniProt ID O15198
Storage Short term: 4°C; Long term: see manual.
Precaution Of Use The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date 8 months
Specificity This assay has high sensitivity and excellent specificity for detection of Mothers Against Decapentaplegic Homolog 9
Cross-Activity No significant cross-reactivity or interference between Mothers Against Decapentaplegic Homolog 9 and analogues was observed.
Assay Procedure 1.Prepare all reagents, samples and standards; 2.Add 100 µL standard or sample to each well. Incubate 2 hours at 37°C; 3.Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4.Aspirate and wash 3 times; 5.Add 100 µL prepared Dete
Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions For research use only. Not for diagnostic procedures.
Quality Systems The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Assurance Biomatik ELISA kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, S
Research Area Signal transduction, Developmental science, Bone metabolism

 

Cat. No. Name Size Price (USD) Qty Add to Cart
EKU10157 Human Mothers Against Decapentaplegic Homolog 9 (Smad9) ELISA Kit 96 Tests $735.00

* Discounts available on orders of 5+ kits.