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Mouse Cholesterol-25-Hydroxylase (CH25H) ELISA Kit

Mouse Cholesterol-25-Hydroxylase (CH25H) ELISA Kit

Cat. No. EKU09230
Product Name Mouse Cholesterol-25-Hydroxylase (CH25H) ELISA Kit
Species Reactivity Mus musculus (Mouse)
Estimated Turnaround Time 6-11 Business days

Product Description

Format 96-Well Plate
Target Name Cholesterol-25-Hydroxylase
Target Synonyms h25OH, Cholesterol 25-monooxygenase
Sample Type Tissue homogenates, Cell lysates and other biological fluids
Detection Range 0.781-50 ng/mL
Sensitivity 0.27 ng/mL
Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholesterol-25-Hydroxylase were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholesterol-25-Hydroxylase were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV<10%;Inter-Assay: CV<12%
Detection Method Colorimetric
Assay Time 3 hours
Application ELISA
Assay Type Double-antibody Sandwich
Shipping Condition Ice packs
UniProt ID Q9Z0F5
Storage Short term: 4°C; Long term: see manual.
Precaution Of Use The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date 8 months
Specificity This assay has high sensitivity and excellent specificity for detection of Cholesterol-25-Hydroxylase
Cross-Activity No significant cross-reactivity or interference between Cholesterol-25-Hydroxylase and analogues was observed.
Assay Procedure 1.Prepare all reagents, samples and standards; 2.Add 100 µL standard or sample to each well. Incubate 2 hours at 37°C; 3.Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4.Aspirate and wash 3 times; 5.Add 100 µL prepared Dete
Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions For research use only. Not for diagnostic procedures.
Quality Systems The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Assurance Biomatik ELISA kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, S
Research Area Enzyme & Kinase

 

Cat. No. Name Size Price (USD) Qty Add to Cart
EKU09230 Mouse Cholesterol-25-Hydroxylase (CH25H) ELISA Kit 96 Tests $756.00

* Discounts available on orders of 5+ kits.