Tissue homogenates and other biological fluids.
Estimated Turnaround Time
7-11 business days
DIDO1 / Death Inducer Obliterator 1
BYE1; DATF1; DIDO2; DIDO3; DIO1; Death Associated Transcription Factor 1
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Short term: 4°C; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
This assay has high sensitivity and excellent specificity for detection of Death Inducer Obliterator 1 (DIDO1). No significant cross-reactivity or interference between Death Inducer Obliterator 1 (DIDO1) and analogues was observed.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1A2 (CYP1A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 1A2 (CYP1A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 1A2 (CYP1A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 1A2 (CYP1A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay procedure summary
1. Prepare all reagents, samples and standards; 2. Add 100µl standard or sample to each well. Incubate 2 hours at 37°C ; 3. Aspirate and add 100µl prepared Detection Reagent A. Incubate 1 hour at 37°C ; 4. Aspirate and wash 3 times; 5. Add 100µl prepared Detection Reagent B. Incubate 30 minutes at 37°C ; 6. Aspirate and wash 5 times; 7. Add 90µl Substrate Solution. Incubate 10-20 minutes at 37°C ; 8. Add 50µl Stop Solution. Read at 450nm immediately.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
For Research Use Only. Not for use in diagnostic procedures. Not for human or animal drug or food use.
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in the kit before experiment.
To edit this page simply login to the control panel, click the Website Content tab and choose the View Web Pages option. Click Edit next to the Shipping & Returns page and you can change this text. A sample returns policy is shown below which you can edit as needed.
You may return most new, unopened items within 30 days of delivery for a full refund. We'll also pay the return shipping costs if the return is a result of our error (you received an incorrect or defective item, etc.).
You should expect to receive your refund within four weeks of giving your package to the return shipper, however, in many cases you will receive a refund more quickly. This time period includes the transit time for us to receive your return from the shipper (5 to 10 business days), the time it takes us to process your return once we receive it (3 to 5 business days), and the time it takes your bank to process our refund request (5 to 10 business days).
If you need to return an item, simply login to your account, view the order using the "Complete Orders" link under the My Account menu and click the Return Item(s) button. We'll notify you via e-mail of your refund once we've received and processed the returned item.
We can ship to virtually any address in the world. Note that there are restrictions on some products, and some products cannot be shipped to international destinations.
When you place an order, we will estimate shipping and delivery dates for you based on the availability of your items and the shipping options you choose. Depending on the shipping provider you choose, shipping date estimates may appear on the shipping quotes page.
Please also note that the shipping rates for many items we sell are weight-based. The weight of any such item can be found on its detail page. To reflect the policies of the shipping companies we use, all weights will be rounded up to the next full pound.
Integer et est tellus non bibendum est. Namcos tempus turpis at metus scelerisque placerat nulla eu sollicitudin felis. Pellentesque diam dolor elementum et lobortis at mollis ut risus. Sed faucibus ullamcorper mattis. Fusce molestie elit a loremos tempus scelerisque blandit tortor cursus. Quisque dolutpat orci ut metus malesuada lorem in interdum lectus scelerisque. Praesent eu odio ut nisi ullamcorper ultricies. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus.
Praesent at justo congue leo adipiscing
Integer et est tellusInteger et est tellus non bibendum est.
Namcos tempusNamcos tempus turpis at metus scelerisque placerat nulla eu sollicitudin felis.
Pellentesque diam dolorPellentesque diam dolor elementum et lobortis at mollis ut risus.
Sed faucibusSed faucibus ullamcorper mattis.
Fusce molestie elitosFusce molestie elit a loremos tempus scelerisque blandit tortor cursus.
Quisque dolutpat orcisQuisque dolutpat orci ut metus malesuada lorem in interdum lectus scelerisque.
Praesent an modioPraesent eu odio ut nisi ullamcorper ultricies.
Cum sociis natoque penatibusCum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus.