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ELISA Kits Overview

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ELISA KITS from Biomatik

Biomatik offers an extensive list of over 22.000 high quality ELISA Kits, shipped typically within 7-11 business days.

An enzyme-linked immunosorbent assay also referred to as ELISA, is a highly sensitive, quantitative method used in the laboratory to assist in the detection of a wide range of analytes. This test measures the concentration of antibodies or antigens and detects their interaction with one another. Antigens are molecules with distinct features, which allow them to stimulate specific responses from the immune system. Once stimulated, the immune system releases an antibody specific to the antigen its responding to, acting like a lock and key combination. The ELISA Kit utilized these analytes for fast detection and amazing specificity.

This test can be performed on a number of different sample types, including:

- Human             - Mouse                   - Monkey                      - Porcine
- Rat                   - Bovine                   - Sheep                        - Goat
- Rabbit              - Chicken                 - Guinea Pig                 - Canine     

 


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Techniques we offer


Sandwich ELISA     Competitive ELISA   Cell-Based ELISA
The ELISA plate is coated with capture antibodies and the remaining surface is blocked with BSA or detergent. The plate is then loaded with a sample in which only the protein of interest binds to the capture antibodies and everything else gets washed away. Next, detection antibodies which are linked to enzymes are added to the plate to bind to existing proteins. Once a colorless substrate (TMB) is added, the enzymes that have found proteins will cause a color change in the substrate. The color intensity is dependent on the concentration of the protein of interest.         The ELISA plate is coated with capture antibodies and the remaining surface is blocked with BSA or detergent. The sample is then mixed with an enzyme conjugated version of the protein of interest. When this mixture is added to the plate, the proteins in the sample will compete with the enzyme conjugates to bind with the limited number of antibodies.  After washing away the excess mixture, a colorless substrate is added to the plate (TMB). If the sample had a high amount of the protein of interest, there would be less enzyme conjugate bound to the plate, which would result in a lower color intensity.​        The ELISA plate is coated with seed cells and allowed to adhere for fixation. A primary antibody is added and allowed to bind before any excess is washed away. Next, a secondary antibody is added to the plate and allowed to bind before any excess is washed away. The target protein or post-translational modification of the target protein are then quantified with an IR scanner or a spectrophotometer (if using an HRP labeled microplate). Cell staining is an optional step for additional data collection to measure relative cell density. 
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