Trusted by 10,000+ Scientists Since 2002. View Our Customer Testimonials, Selected Publications and 3,000+ Cited Publications at Google Scholar.

Peptide Synthesis

custom-peptide-synthesis

Custom Peptide Synthesis Service

  peptide_synthesis_quote_request 

Select publications featuring custom peptides synthesized by Biomatik:

Peptide Synthesis Service Highlights

        • Over 99.5% success rate with "No Peptide, No Charge" policy.
        • As low as $3/aa or $90/peptide for custom peptide synthesis services. As low as $25/peptide for peptide library services.
        • From crude to 99% purity; from milligrams to kilograms.
        • Wide range of peptide modifications (250+) are available. Click Peptide Modifications tab below or download here
        • Fast turnaround time - most peptides under 30aa are delivered within 2-3 weeks.
        • One stop for custom gene, peptide, protein and antibody services. 

Biomatik has been proudly serving 10,000+ scientists since 2002. Biomatik is proud to offer high quality and speedy peptide synthesis service at the most affordable prices in the industry: as low as $3/aa or $90/peptide, and as fast as 2-3 weeks for most peptides under 30aa!

Our extensive experience in peptide synthesis technologies has afforded us the capability to take on a wide range of sequences, purity levels, and modifications to meet your research needs. Our high success rate means you will get your custom peptide made so you can move forward with your research goals. If we fail to synthesize the peptide at the specified quantity and purity, there is absolutely no charge to you. We offer this same guarantee regardless of peptide complexity!

LengthPurityStarting PriceTurnaround TimePromotional
up to 130aa Crude $3.00/aa ∼2 weeks up to 25% off
up to 130aa >75% $7.50/aa 2-3 weeks up to 25% off
up to 130aa >85% $10.10/aa 2-3 weeks up to 25% off
up to 130aa >90% $11.90/aa 2-3 weeks up to 25% off
up to 130aa >95% $13.00/aa 2-3 weeks up to 25% off
up to 130aa >98% $19.40/aa 2-3 weeks up to 25% off

•  Prices and discounts may vary depending on the peptide length, complexity and the order volume.
•  Minimum $90/peptide. 
All purified peptides are delivered with complete QC data, including HPLC, MS.
•  Low price guarantee - we will match/beat any reputable competitor's price.

Peptide Modifications from Biomatik

We are proud to offer over 250 peptide modifications at very affordable prices. You may click Peptide Modifications tab above or download here.

Here are some popular modifications: Acetylation, Amidation, Biotin, FITC, FAM, Phosphorylation, Cyclization (Disulfide Bonds), Isotope Labeled Amino Acids, Stapled Peptides, CMK, FMK, Spacers (PEGylation, Amino Hexanoic Acid), KLH or BSA Conjugations.

Biomatik also provides Glycopeptide modifications, such as Ser/Thr (GlcNAc), Ser/Thr (GalNAc),Ser/Thr (Gal-GalNAc), etc.

Why Choose Biomatik?

Biomatik has been trusted among top scientists and researchers from around the world for quality products and custom services since 2002. To date, Biomatik has delivered 70,000+ custom made products to our customers worldwide. Learn why Biomatik has been trusted by thousands of researchers worldwide by viewing our customer testimonials, selected publications and 3,000+ publications citing our fine products and services at Google Scholar.

"I have been ordering peptides (both with or without modifications) from Biomatik over and over in the past two years. Every single time the process is extremely smooth - thanks to their great customer service team and production team! Also the prices are just unbeatable. I will definitely choose Biomatik over other companies for all my peptide orders."  
-- Dr. Tam, University of California Berkeley - Optometry, Berkeley, CA, USA

"Easy-to-obtain prompt quotes online, reasonable prices, very responsive service, and high quality peptides. I will continue to use Biomatik and recommend them to colleagues." 
-- Dr. Napper, Nemours Center for Childhood Cancer Research, Wilmington, DE, USA

Peptide Synthesis Service Knowledge Base

Peptide Synthesis Quotation and Ordering

Our peptide synthesis quotation request and ordering processes involve 3 simple steps:
1. Submit your custom peptide sequence information and specifications online.
2. Our Technical Support personnel will provide a formal project price quote and answer any technical questions.
3. Initiate production by providing a formal PO or pay with credit card through our online ordering system.

 

Peptide Modifications

Last updated: Oct 10, 2020

N terminus Modification
H (free amine)
Ac (Acetylation)
Fmoc
CBZ
Bz
Bz(4-F)
Bz(4-NO2)
Pyr
D-Pyr
LA-
Mpa-
Mal-b-Ala
Mal-Acp
Aoa

N terminus Fatylation
For (Formylation)
2-Br-Ac
2-Cl-Ac
2-I-Ac
OH-Ac-
But-
Suc
MeOSuc
Iba
Hex-
5-heptenoic acid
5-Hexynoic acid
heptanedioic acid
Oct-
Dec-
Sebacic acid
Lau-
Myr-
Pal-
Ste-
Octanedioic acid
Oleic Acid-

C terminus Modification
-OH (free acid)
-NH2 (Amidation)
Cysteamide, -Cya
-AMC
-OMe
-OEt
-OBzl
-OtBu
-NHMe
-NHEt
-TBzl
p-Nitroanilide

D form normal amino acid
{D-Ala}
{D-Arg}
{D-Asp}
{D-Asn}
{D-Cys}
{D-Glu}
{D-Gln}
{D-His}
{D-Allo-Ile}
{D-Ile}
{D-Leu}
{D-Lys}
{D-Met}
{D-Pro}
{D-Phe}
{D-Ser}
{D-Tyr}
{D-Thr}
{D-Trp}
{D-Val}

Unusual amino acid
{Beta-Asp}
{D-Beta-Asp}
{Gamma-Glu}
{D-Gamma-Glu}
Cys(Cam)
D-Cys(Cam)
{Cys(Acm)}
{Cys(tBu)}
{Met(O)}
{D-Met(O)}
{Met(O)2}
{D-Met(O)2}
{Lys(Ac)}
{Ac-Lys}
{Lys(Dde)}
{Tle}
{D-Ser(octanoic acid)}
2-Thi
3-Thi
{Aib}
{Abu}
{D-Abu}
{Hyp}
{Phg}
{D-Phg}
{Nva}
{D-Nva}
{Norleucine}
{D-Nle}
{Cit}
{D-Cit}
{Orn}
{D-Orn}
{Pen}
{D-Pen}
{Cpg}
{Cha}
{D-Cha}
{Chg}
{D-Chg}
{Dab}
{Dap}
Pra
D-Pra
Allo-Thr
D-Allo-Thr
{D-1-Nal}
{L-1-Nal}
{D-2-Nal}
{L-2-Nal}
{D-2-Pal}
{L-2-Pal}
{D-3-Pal}
{L-3-Pal}
{D-4-Pal}
{L-4-Pal}
{Oic}
{Tic}
{D-Tic}
X (20 kinds amino acids mixture with equal mol)

Fluorescence/Dye labeling
Biotin-
-Lys(Biotin)-
-Lys(Biotin)
Biotin-Ahx-
FITC-
-Lys(FITC)-
-Lys(FITC)
FITC-Ahx-
5-FAM-
5,6-FAM
6'FAM
-Lys(5-FAM)-
Lys(5,6-FAM)
-Lys(5-FAM)
5-FAM-Ahx-
Dansyl-
-Lys(Dansyl)-
-Lys(Dansyl)
Dansyl-Ahx-
5-TAMRA-
5(6)-TAMTA-
-Lys(TAMRA)-
-Lys(5-TAMRA)
{5-TAMRA-Acp}
{Lys(Dnp)}
{D-Lys(Dnp)}
{Dab(Dnp)}
Dap(Dnp)
MCA-
-Lys(MCA)-
-Lys(MCA)
Rhodamine B-
Lys(Rhodamine B)

Phe/Tyr Analogs amino acid
{D-2-Cl-Phe}
{L-2-Cl-Phe}
{D-3-Cl-Phe}
{L-3-Cl-Phe}
{D-4-Cl-Phe}
{L-4-Cl-Phe}
{D-3,4-DiCl-Phe}
{L-3,4-DiCl-Phe}
{D-4-Br-Phe}
{L-4-Br-Phe}
{D-3-F-Phe}
{L-3-F-Phe}
{D-4-F-Phe}
{L-4-F-Phe}
{D-4-NO2-Phe}
{L-4-NO2-Phe}
{D-4-I-Phe}
{L-4-I-Phe}
{D-3-CN-Phe}
{L-3-CN-Phe}
{D-4-CN-Phe}
{L-4-CN-Phe}
{D-2-Me-Phe}
{L-2-Me-Phe}
{D-4-Me-Phe}
{L-4-Me-Phe}
{D-4-NH2-Phe}
{L-4-NH2-Phe}
{D-3-Cl-Tyr}
{L-3-Cl-Tyr}
{D-3,5-DiCl-Tyr}
{L-3,5-DiCl-Tyr}
{D-3,5-DiBr-Tyr}
{L-3,5-DiBr-Tyr}
{D-3-I-Tyr}
{L-3-I-Tyr}
{D-3,5-DiI-Tyr}
{L-3,5-DiI-Tyr}
{D-3-NO2-Tyr}
{L-3-NO2-Tyr}
{D-3,5-DiNO2-Tyr}
{L-3,5-DiNO2-Tyr}
{L-3-F-Tyr}

Homo amino acid
{Har}
{D-Har}
{HomoCit}
{D-HomoCit}
{HomoLeu}
{HomoPro}
{D-HomoPro}
{beta-HomoIle}
{beta-HomoLeu}
{beta-HomoMet}
{beta-HomoPro}
{beta-HomoVal}
Quenched fluorescent peptide
{Abz}
o-Abz- (o-aminobenzoic acid)
{Tyr(3-NO2)}
{Glu(EDANS)}
{DABCYL}
{Lys(DABCYL)}
{Lys(Abz)}
MAPS and Carrier Complex
{Symmetric 2 Branches}
{Symmetric 4 Branches}
{Symmetric 8 Branches}
Lys(Maleimide)
Lys(Mpa)
Lys(Pra)
Lys(Suc)
Lys(glutaryl)
Lys(pGlu)
Lys(For)
Lys(2-Br-Ac)
Lys(Butanoyl)
Lys(Crotonyl)
{Lys(octenyl)}
lys(Ma)
Lys(Pal)
Lys(Oleic Acid)
Lys(Acryl)
Lys(alkine)
Lys(Alloc)
Lys(Aoa)
Lys(cyclopropanecarboxyl)
{Lys(3,5-diiodo-4-hydroxybenzoyl)}
Lys(Methacryl)
Lys(propargyl)
Lys(propionyl)
Lys(Pyruvoyl)
{BSA-Peptide N terminus}
{BSA-peptide C terminus}
{BSA-Peptide via Cys}
{KLH-Peptide N terminus}
{KLH-Peptide C terminus}
{KLH-peptide via Cys}
{OVA-Peptide N terminus}
{OVA-peptide C terminus}
{OVA-peptide via Cys}

Atom Linker
{betaAla}
{Ava}
{Ahx}
{8-Aoc}
{AEA}
{Ado}
{ANP Linker}

Methyl amino acids
{Lys(Me)}
{Lys(Me2)}
{Lys(Me3)}
N-Methyl amino acid
{N-Me-Ala}
{N-Me-Phe}
{N-Me-Leu}
{N-Me-Ile}
{N-Me-Val}
{N-Me-Met}
{N-Me-Nle}
{N-Me-Nva}
{Sar}
{N-Me-Ser}
{N-Me-Tyr}
{N-Me-Thr}
{N-Me-Asp}
{N-Me-Glu}
N-Me-beta-Ala

Cyclic peptide
{Mono Disulfide bridge}
{Double Disulfide bridge}
{Triple Disulfide bridge}
{Random Disulfide bridge}
{Same Seq. Inter-Disulfide bridge }
{Different Inter-Disulfide bridge}
{Amide cyclic (end)}

Phosphorylation
{pSer}
{pTyr}
{pThr}
{D-pSer}
{D-pTyr}
{D-pThr}
{Di-sites in sequence}
{Tri-sites in sequence}

PEG
{Mini-PEG}
{Mini-PEG2}
{Mini-PEG3}
{PEG4}
{PEG-6}
{PEG8}
{PEG-11}
{PEG-12}

Isotope Labeling
H2 (deuterium)
N15
C13
13C15N-K (C-terminal)
13C15N-R (C-terminal)
13C15N-A (C-terminal)
13C15N-I (C-terminal)
13C15N-L (C-terminal)
13C15N-F (C-terminal)
13C15N-P (C-terminal)
13C15N-V (C-terminal)

Stapled Peptides
Single stapled S5 and S5
Single stapled S5 and R8
Single stapled R8 and R8
Double stapled S5 and S5
Double stapled S5 and R8
Double stapled R8 and R8

Glycopeptides (glycosylated peptides)
N-linked glycopeptide synthesis
O-linked glycopeptide synthesis
C-linked glycopeptide synthesis
S-linked glycopeptide synthesis
Glycopeptides containing monosaccharides, such as Ser/Thr (GlcNAc), Ser/Thr (GalNAc), Asn (GlcNAc), Ser (Xyl), Thr (Man), etc.
Glycopeptides containing oligosaccharides, such as Ser/Thr (Gal-GalNAc), Ser/Thr (Neu-Gal-GalNAc), Asn (Fuc-GlcNAc), etc.


Misc
FMK, CMK
N6-Octanoyl
Photocleavable peptides
propargyl-gly
Lys(Myr)
pNA
Lys(N3)


 

Peptide Synthesis Introduction

Peptide synthesis is the chemical process of joining two or more amino acids together via peptide bonds. Typical amide bonds are formed by the condensation reaction of the carboxyl group of one amino acid to the amino group from the growing peptide chain. Chemical peptide synthesis occurs from the C- to the N-terminus direction, and it is a precise step-wise addition of one amino acid at a time. In solid-phase synthesis, the process begins by first anchoring to a resin polymer at the C-terminal end, leaving the N-terminal amine unit free to attach to another N-protected amino acid unit. This newly added amino acid will then undergo deprotection of the N-terminal amine thereby allowing the addition of another N-protected amino acid unit. The assembling continues by repeating this cycle of coupling, washing, and deprotection reactions. Once complete, the final peptide is cleaved from the anchoring resin and purified.

Synthetic Peptide Applications

Synthetic peptides can be used in a wide variety of applications and research areas including disease biomarker identification, drug target development, cancer diagnosis and therapeutics, epitope mapping, antibody productions, protein functionality and characterizations, enzyme-substrate or receptor-substrate interactions, and vaccine design to name a few.

Peptide Synthesis FAQs

Below are some frequently asked questions about the Biomatik Peptide Synthesis service. If you have any other questions, please contact us at Peptide@biomatik.com and we will be happy to assist you.

1. What is the typical turn-around time for peptide synthesis? How do you ship and how long does it take to ship to me?
Our typical turn-around time is 2-3 weeks for a standard peptide under 30 amino acids. The turn-around time varies depending on the peptide length, solubility and difficulty. All peptides will be shipped in lyophilized powder, at room temperature. It is overnight delivery within USA/Canada, and takes typically 3-4 days to reach researchers in other countries.

2. Which analytical data do you provide for peptides?
Each peptide synthesis comes with a project report (CoA). Each synthetic peptide comes with the following analytical data:  
Appearance 
Molecular formula
Theoretical molecular mass
Molecular mass, obtained by
mass spectrometry 
Purity, determined by HPLC
Peptide content analysis 
Solubility test 

We can provide additional analysis if required, e.g. Net Peptide content analysis, TFA content analysis, and KF test (Water content test).
3. Which purity is recommended for my application?
>75%, preferably >85%: immunological applications, polyclonal antibody production and non-sensitive screening
>90%: SAR studies, bioassays
>95%: In vitro bioassays such as ELISA, enzymology, biological activity
>98%: Structural studies such as Crystallography, NMR or sensitive bioassays
Download our Recommended Peptide Purity Guideline for more details.

4. TFA salt form vs. Acetate or HCl salt form: Which form should I choose?
By default, all research peptides are synthesized in TFA salt form. For cell based assays or animal studies, you shall consider having TFA salt removed by switching it to acetate or HCl salt form (with TFA <1% guarantee) to avoid abnormal responses. Depending on the budget, you may also want to consider higher purities (>98%) to get optimal results.

5.How long you can synthesize a peptide?
Biomatik can synthesize peptides up to 130aa. Unlike many peptide suppliers who are only comfortable in making peptides under 30 or 40aa, Biomatik has extensive experience in making peptides ranging from 40aa to 90aa. The success rate is much lower when it comes to longer peptides, especially 100aa or longer. If you plan to synthesize a sequence of 130aa or longer, please contact us for custom protein expression and purification service.

6. What type of end terminal modification choice is appropriate?
By default, chemically synthesized peptides have free amine at N-terminal and free acid at C-terminal. N-terminal acetylation and C-terminal amidation are uncharged, which reduces the overall charge of a peptide so the solubility may decrease. However, the modifications are desirable since it imitates its natural structure. It increases the metabolic stability of peptides and their ability to resist enzymatic degradation by aminopeptidases, exopeptidases, and synthetases. This enhances their ability to enter cells, thus increasing the biological activity of a peptide.
We recommend the modifications for intracellular, in-vivo assays and in-vitro functional studies. The modified peptides can then be used as substrates in enzyme assays. Amidation not only enhances the activity of peptide hormones, it also prolongs their shelf life. The modifications can reduce the influence of charged C- or N-terminal during ELISA binding assays.

7. How to dissolve my peptide?
Upon delivery, we will include a Peptide Handling Guideline which will help you in dissolving your peptide properly. You may request peptide solubility test at the time of ordering, free of charge.
8. How do I store my synthetic peptides?
Most lyophilized peptides will be stable at room temperature for 2-3 weeks. For long-term storage, you should store lyophilized peptides at -20°C. Repeated freeze-thaw cycles should be avoided. Allow to come to room temperature before opening. The shelf life of peptide solutions is limited; a peptide solution once prepared should be used as soon as possible.

9. What if some problems come up during the synthesis or purification process?
Each peptide has its specific characteristics. If some problems happen during the synthesis beyond our expectation, and we cannot deliver your peptide on time, we will inform you as soon as possible. By chance that we are not able to make the peptide, you will not be charged for any costs.

10. What is the appropriate peptide length for antibody production?
Generally, a 10-25 residue peptide is recommended. A longer peptide could have more epitopes, but could also have a greater chance of forming stable secondary structures which are not native forms. A shorter peptide (<10aa) is generally not good unless there are valid reasons for it, such as potential sequence homology with a related family member or other proteins. Biomatik also offers custom antibody production service, please contact us for more details or check out our Antibody Production Service page for information.

11. How to determine net weight and molar amount of the desired target peptide?
Biomatik ships peptides according to the gross weight of lyophilized powder. The lyophilized powder includes impurities such as fragment peptides, salts and residual water. Please refer to our Peptide Handling Guideline for determining the molar calculation of your synthetic peptide concentration.

12. How do I design custom peptide sequence for my applications?
The sequence, amino acid composition and length of a peptide will influence whether correct assembly and purification are feasible. These factors also determine the solubility of the final product. Please refer to our Peptide Design Guideline on some factors in the design of a peptide sequence for synthesis.

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order. Orders are typically confirmed within 24 hours.
peptide_synthesis_quote_request

  • Have a Question? Email us
  • Order Online: Credit Card Order Form
  • By Fax: 1-877-221-3515 (USA/Canada) or 1-519-231-0140 (International)
  • By Phone: 1-800-836-8089 (USA/Canada) or 1-519-489-7195 (International)

 

 

 

Top