Is codon optimization included in Biomatik’s gene synthesis services?
Yes. To achieve stable and enhanced heterologous protein expression levels, Biomatik offers complementary codon optimization service to improve codon usage in one or multiple host cell systems. This powerful tool combines our proprietary optimization algorithm and synthetic DNA technologies to achieve the highest possible expression level in any given host system.
What is Gene Synthesis?
Gene synthesis is a powerful technique that enables researchers to design and produce tailor-made DNA sequences without the need for templates or PCR amplification. Unlike traditional cloning, synthetic genes are built from scratch using chemically synthesized oligonucleotides, providing maximum flexibility in gene design, codon optimization, and sequence editing. We also offer a wide range of downstream services, including subcloning, mutagenesis, and plasmid preparations.
This flexibility enables a wide range of applications, including:
-Codon-optimized genes for expression in various hosts
-Mutant libraries and variant constructs
-Hard-to-clone or GC-rich sequences
-Synthetic biology and vaccine research
-Plasmid construction and subcloning
Can Biomatik perform site-directed mutagenesis?
Absolutely. Our gene synthesis services include support for site-directed mutagenesis, allowing you to introduce specific mutations, deletions, or insertions as part of your construct design.
What vectors are available for cloning?
We offer a wide selection of over 120 vectors for cloning. Genes are typically delivered in a standard cloning vector, but we can subclone into your preferred vector upon request.
How is the gene delivered?
Each order includes plasmid preparation, providing 4µg of lyophilized plasmid DNA containing your synthetic gene insert. Additional quantities or alternative formats can be arranged if required.
What quality control measures are in place?
All genes undergo full sequence verification by Sanger sequencing to ensure 100% accuracy. A detailed QA report is provided with every delivery.
Can Biomatik handle complex or GC-rich sequences?
Yes, as a flexible gene synthesis CRO, we have extensive experience synthesizing difficult constructs, including GC-rich sequences and repetitive regions.
Please can you provide information on your pricing and turnaround times (TAT)?
Pricing and turnaround times are dependent on gene length. Summarized in the table below.
| Gene Length |
TAT |
List Price |
Special Offer |
| Up to 1.5 kb |
2 weeks |
$0.20/bp |
Volume discount available |
| 1.5-3 kb |
2-3 weeks |
$0.20/bp |
Volume discount available |
| 3-5 kb |
3-5 weeks |
$0.24/bp |
Volume discount available |
| 5+ kb |
Please inquire |
Discounts may vary depending on the gene length, complexity and the order volume. Minimum $150/gene. Turnaround time is an estimated time only; it does not reflect any production difficulties, if encountered.
