What do you need for sequencing?
We require 200µg of monoclonal antibody at ≥90% purity for sequencing. If you require recombinant antibody production, we also need 50µg of antigen ≥90% purity for ELISA detection.
Can you identify all amino acids, including isoleucine and leucine?
Yes. With 200µg of antibody at ≥90% purity, we can identify all amino acids and accurately distinguish between isoleucine and leucine.
What is your sequencing accuracy and coverage?
We guarantee 100% accuracy and full coverage of the desired VH and VL domains.
How long does the sequencing process take?
Standard sequencing turnaround is as fast as 2–3 weeks.
Can you produce a recombinant antibody from the sequence?
Once sequencing is complete, we can produce a recombinant antibody with binding affinity verified by ELISA.
What is included in the antibody sequencing and recombinant antibody production package?
The full package includes antibody sequencing with a 100% coverage guarantee, followed by recombinant antibody production and binding affinity detection by ELISA. You will receive antibody sequencing data, a coverage map, 1 mg of recombinant antibody at ≥90% purity with ELISA-verified binding, and 4µg of plasmid containing the gene insert. You can find more information about our custom recombinant antibody production here.
Do you offer a guarantee for your services?
Yes. Our risk-free guarantee includes a guaranteed antibody sequencing result – or it’s free of charge
Please can you provide details of your pricing and packages?
Absolutely. You can download our packages, including estimated turnaround times, here