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Cat#

RPU60417
List Price:
$345.60

 

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Technical Documents:

Target Name

Insulin Like Growth Factor 1

Target Synonyms

IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor

Species

Human (Homo sapiens)

Host

293F Cell

Protein Type

Active Protein

Tag

N-Terminal His Tag and C-Terminal Fc Region of Human IgG1

Activity

Biologically Active

Applications

Cell Culture, Activity Assays

tid

50ug,345.6;100ug,483.8;1mg,2246.4

Human Insulin Like Growth Factor 1, Active Protein is a purified Active Protein. Purity: >80% by SDS-PAGE. Host: 293F Cell. Endotoxin Level: <1.0EU per 1ug, by the LAL method. Species: Human (Homo sapiens). Target Name: Insulin Like Growth Factor 1. Accession Number: P05019. Gene ID: 3479. Expression Region: Gly49~Ala118. Tag Info: N-terminal His Tag and C-terminal Fc Region of Human IgG1. Theoretical MW: 50kda. Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.. Target Synonyms: IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Lead Time

7-11 Business Days

Expression System

Eukaryotic expression

Accession Number

P05019

Gene ID

3479

Expression Region

Gly49~Ala118

Theoretical MW

50kDa

Conjugate

Unconjugated

Endotoxin Level

<1.0EU/ug, by the LAL method

Isoelectric Point

7.7

Applications

Cell Culture, Activity Assays

Purity

>80% by SDS-PAGE

Format

Freeze-dried powder

Storage

Store at 2-8°C for one month,-80°C for 12 months

Buffer

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Expiration

Stable for 12 months if correctly stored

Reconstitution

Refer to the datasheet/CoA included in the product pouch.

Shipping Condition

Ice packs

Research Area

Tumor Immunity

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 and ISO 13485 certified facilities.

Technical Documents

Selected Citations Featuring Biomatik's Products & Services
Last updated 2020-01-31

 

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SDS-PAGE
Insulin-like growth factor 1 (IGF1), also called somatomedin C is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF1, mecasermin, is used for the treatment of growth failure. IGF1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. Besides, Insulin Like Growth Factor Binding Protein 2 (IGFBP2) has been identified as an interactor of IGF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IGF1 and recombinant mouse IGFBP2. Briefly, IGF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IGFBP4-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IGF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human IGF1 and recombinant mouse IGFBP2 was shown, the EC50 for this effect is 0.087 ug/mL.

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