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Human Peptidylglycine Alpha Amidating Monooxygenase, Active Protein, Cat#RPU60672

Cat#

RPU60672
List Price:
$897.60

 

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Technical Documents:

Target Name

Peptidylglycine Alpha Amidating Monooxygenase

Target Synonyms

PAL; PHM; Peptidylamidoglycolate lyase; Peptidyl-Alpha-hydroxyglycine Alpha-amidating Lyase; Peptidylglycine Alpha-Hydroxylating Monooxygenase

Species

Human (Homo sapiens)

Host

293F cell

Protein Type

Active Protein

Tag

N-Terminal His Tag

Activity

Biologically Active

Applications

Cell Culture, Activity Assays

tid

50ug,897.6;100ug,1256.6;1mg,5834.4

Human Peptidylglycine Alpha Amidating Monooxygenase, Active Protein is a purified Active Protein. Purity: >90% by SDS-PAGE. Host: 293F cell. Endotoxin Level: <1.0EU per 1ug, by the LAL method. Species: Human (Homo sapiens). Target Name: Peptidylglycine Alpha Amidating Monooxygenase. Accession Number: P19021. Gene ID: 5066. Expression Region: Phe31~Val817. Tag Info: N-terminal His Tag. Theoretical MW: 70kda. Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.. Target Synonyms: PAL; PHM; Peptidylamidoglycolate lyase; Peptidyl-Alpha-hydroxyglycine Alpha-amidating Lyase; Peptidylglycine Alpha-Hydroxylating Monooxygenase Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Lead Time

7-11 Business Days

Expression System

Eukaryotic expression

Accession Number

P19021

Gene ID

5066

Expression Region

Phe31~Val817

Theoretical MW

70kDa

Conjugate

Unconjugated

Endotoxin Level

<1.0EU/ug, by the LAL method

Isoelectric Point

6.1

Applications

Cell Culture, Activity Assays

Purity

>90% by SDS-PAGE

Format

Freeze-dried powder

Storage

Store at 2-8°C for one month,-80°C for 12 months

Buffer

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Expiration

Stable for 12 months if correctly stored

Reconstitution

Refer to the datasheet/CoA included in the product pouch.

Shipping Condition

Ice packs

Research Area

Enzyme, Kinase

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 and ISO 13485 certified facilities.

Technical Documents

Selected Citations Featuring Biomatik's Products & Services
Last updated 2020-01-31

 

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SDS-PAGE
Peptidyl-glycine alpha-amidating monooxygenase (PAM) is an enzyme that is required for the biosynthesis of many signaling peptides. It has two enzymatically active domains with catalytic activities-peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. A typical activity assay was using Dns-Tyr-Val-Gly as substrate, thus the activity of recombinant human PAM was measured by its ability to hydrolyze Dns-Tyr-Val-Gly to Dns-Tyr-Val-NH2. The reaction was performed in 100 mM MES/KOH pH 6.0, 30 mM KI, 30 mM KCl, 1 uM cupric sulfate, 100 ug/ml catalase (APC418Hu05), 1% (v/v) ethanol, 0.001% (v/v) Triton X-100 and 10 mM ascorbate. 250 ul 0.7 mM substrate of Dns-Tyr-Val-Gly was added with 250 ul various concentrations of recombinant human PAM (1 ug/ml,5 ug/ml, 10 ug/ml and 20 ug/ml). Incubated at 37°C for 30min, the reaction was stopped by addition 6% (v/v) TCA. The product and substrate was detected by RP-HPLC with UV-detection at 280 nm, the analyses were performed at 25°C employing a Agilent ZORBAX Poroshell SB C18 column (9.4×250 mm, 5 μm), the flow rate was 1 ml/min. The mobile phase consisted of 100 mM sodium acetate (pH 6.5) and 30 min linear gradient of 10-90% acetonitrile. At 30-35 min, the mobile phase consisted of 90% acetonitrile and 10% sodium acetate. The substrate have been hydrolyzed to Dns-Tyr-Val-NH2 after incubating with recombinant human PAM. The retention time of Dns-Tyr-Val-Gly and Dns-Tyr-Val-NH2 is 24.088 and 30.421 respectively. The specific activity of recombinant human PAM is >6600 pmol/min/ug.
The reaction product compared with standard Dns-Tyr-Val-Gly and Dns-Tyr-Val-NH2.
The standard curve of Dns-Tyr-Val-NH2
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