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Human Prostate Specific Antigen, Active Protein, Cat#RPU60276

Cat#

RPU60276
List Price:
$658.60

 

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Technical Documents:

Target Name

Prostate Specific Antigen

Target Synonyms

KLK3; APS; KLK2A1; HK3; KLKB1; Seminin; Prostate Specific Antigen; Kallikrein-Related Peptidase 3; Semenogelase, g-Seminoprotein; P-30 Antigen; Plasma kallikrein

Species

Human (Homo sapiens)

Host

E. coli

Protein Type

Active Protein

Tag

N-Terminal His Tag

Activity

Biologically Active

Applications

Cell Culture, Activity Assays

tid

50ug,658.6;100ug,922.3;1mg,4280.6

Human Prostate Specific Antigen, Active Protein is a purified Active Protein. Purity: >90% by SDS-PAGE. Host: E. coli. Endotoxin Level: <1.0EU per 1ug, by the LAL method. Species: Human (Homo sapiens). Target Name: Prostate Specific Antigen. Accession Number: P07288. Gene ID: 354. Expression Region: Ile25~Pro261. Tag Info: N-terminal His Tag. Theoretical MW: 30kda. Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.. Target Synonyms: KLK3; APS; KLK2A1; HK3; KLKB1; Seminin; Prostate Specific Antigen; Kallikrein-Related Peptidase 3; Semenogelase, g-Seminoprotein; P-30 Antigen; Plasma kallikrein Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Lead Time

7-11 Business Days

Expression System

Prokaryotic expression

Accession Number

P07288

Gene ID

354

Expression Region

Ile25~Pro261

Theoretical MW

30kDa

Conjugate

Unconjugated

Endotoxin Level

<1.0EU/ug, by the LAL method

Isoelectric Point

7.5

Applications

Cell Culture, Activity Assays

Purity

>90% by SDS-PAGE

Format

Freeze-dried powder

Storage

Store at 2-8°C for one month,-80°C for 12 months

Buffer

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Expiration

Stable for 12 months if correctly stored

Reconstitution

Refer to the datasheet/CoA included in the product pouch.

Shipping Condition

Ice packs

Research Area

Tumor Immunity, Reproductive Science

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 and ISO 13485 certified facilities.

Technical Documents

Selected Citations Featuring Biomatik's Products & Services
Last updated 2020-01-31

 

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SDS-PAGE
Kallikrein 3, commonly known as prostate specific antigen (PSA), is a serine protease of the human tissue Kallikrein gene family. PSA is synthesized in the ductal and acinar epithelium of the prostate gland and secreted into the seminal plasma in high concentrations (0.5 - 2 g/L). A small portion of PSA “leaks” into the systemic circulation, the levels of which increase significantly (30-fold) from prostate cancer tissue than normal prostate tissue. PSA has become a well established tumor marker that aids the diagnosis, staging, and follow up of prostate cancer. The activity of recombinant human KLK3 is measured by its ability to cleave a colorimetric peptide substrate Suc-Arg-Pro-Tyr-pNa in the assay buffer 50 mM Tris, 1 M NaCl, pH 8.0. The rhKLK3 is diluted to 200 ug/ml in activation buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35, pH 7.5, then activated with a final concentration of 1 ug/ml Thermolysin at 37 °C for 5min. Adding a final concentration of 10 mM 1,10 Phenanthroline to stop the activation. The activated rhKLK3 is diluted to 50 ug/mL in assay buffer. Loading into a clear well plate 50 uL of 50 ug/mL rhKLK3 and start the reaction by adding 50 uL of 2 mM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhKLK3. Then read at 405 nm in kinetic mode for 5 minutes. The specific activity of recombinant human KLK3 is > 400 pmol/min/ug.
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