![Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD2 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris(hydroxymethyl)amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measued the activity of recombinant human SOD2. The reaction was performed in adding 8 μl 5 mmol/L pyrogallol to 200 μl 50mmol/L Tris-HCl, rapidly mixing at 25 °C, then read at 325 nm (using 50mmol/L Tris-HCl as blank control) in kinetic mode for 3 minutes using a microplate reader controlling the pyrogallol autoxidation rate at 0.70 OD/min. Different concentrations of recombinant human SOD2 were added into 200 μl 50 mmol/L Tris-HCl, incubated for 20 min at 25 °C, then adding 8 μl 5 mmol/L pyrogallol to each well, rapidly mixing and read at 325 nm in kinetic mode for 3 minutes. Under these conditions, the enzyme amount of 50% inhibition of pyrogallol autooxidation per minute is defined as a unit. The specific activity of recombinant human SOD2 is 142.2 U/mg.](https://cdn11.bigcommerce.com/s-wsdgwo7qjr/images/stencil/500x659/products/469318/237195/RPU60500_Image_2__41123.1736878233.jpg?c=2 "Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD2 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris(hydroxymethyl)amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measued the activity of recombinant human SOD2. The reaction was performed in adding 8 μl 5 mmol/L pyrogallol to 200 μl 50mmol/L Tris-HCl, rapidly mixing at 25 °C, then read at 325 nm (using 50mmol/L Tris-HCl as blank control) in kinetic mode for 3 minutes using a microplate reader controlling the pyrogallol autoxidation rate at 0.70 OD/min. Different concentrations of recombinant human SOD2 were added into 200 μl 50 mmol/L Tris-HCl, incubated for 20 min at 25 °C, then adding 8 μl 5 mmol/L pyrogallol to each well, rapidly mixing and read at 325 nm in kinetic mode for 3 minutes. Under these conditions, the enzyme amount of 50% inhibition of pyrogallol autooxidation per minute is defined as a unit. The specific activity of recombinant human SOD2 is 142.2 U/mg.")
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Human Superoxide Dismutase 2, Mitochondrial, Active Protein is a purified Active Protein. Purity: >90% by SDS-PAGE. Host: E. coli. Endotoxin Level: <1.0EU per 1ug, by the LAL method. Species: Human (Homo sapiens). Target Name: Superoxide Dismutase 2, Mitochondrial. Accession Number: P04179. Gene ID: 6648. Expression Region: Lys25~Lys222. Tag Info: N-terminal His Tag. Theoretical MW: 26kda. Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.. Target Synonyms: IPO-B; MNSOD; Mn-SOD Restrictions: For Research Use Only. Not for use in diagnostic procedures.
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Selected Citations Featuring Biomatik's Products & Services
Last updated 2020-01-31
- Nature Communications, 10(1): 3667. (2019). PMID: 31413255
Title: PTPσ inhibitors promote hematopoietic stem cell regeneration
Product/Service Type: ELISA Kits - Immunity, 50(2): 446-461.e9. (2019). PMID: 30709742
Title: Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity
Product/Service Type: Biochemicals - Science, 364(6436): pii: eaav0748. (2019). PMID: 30975858
Title: Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides
Product/Service Type: Custom Gene Synthesis Service - Nature, 577: 689-694. (2020). PMID: 31942068
Title: VEGF-C-driven lymphatic drainage enables immunosurveillance of brain tumours
Product/Service Type: Custom Peptide Synthesis Service - Neuron, 102(2): 420-434.e8. (2019). PMID: 30826183
Title: Distinct Modes of Presynaptic Inhibition of Cutaneous Afferents and Their Functions in Behavior
Product/Service Type: Custom Antibody Production Service - PNAS, 115(51): E12005-E12014. (2019). PMID: 30509983
Title: Mycoplasma promotes malignant transformation in vivo, and its DnaK, a bacterial chaperone protein, has broad oncogenic properties
Product/Service Type: Custom Protein Expression & Purification Service
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