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Cat#

RPU60687
List Price:
$835.20

 

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Technical Documents:

Target Name

Complement Component 1, R Subcomponent

Target Synonyms

C1-r; complement Component 1; Complement component 1 subcomponent r

Species

Mouse (Mus musculus)

Host

293F cell

Protein Type

Active Protein

Tag

N-Terminal His Tag

Activity

Biologically Active

Applications

Cell Culture, Activity Assays

tid

50ug,835.2;100ug,1169.3;1mg,5428.8

Mouse Complement Component 1, R Subcomponent, Active Protein is a purified Active Protein. Purity: >90% by SDS-PAGE. Host: 293F cell. Endotoxin Level: <1.0EU per 1ug, by the LAL method. Species: Mouse (Mus musculus). Target Name: Complement Component 1, R Subcomponent. Accession Number: Q8CG16. Gene ID: 50909. Expression Region: Met1~Asn707. Tag Info: N-terminal His Tag. Theoretical MW: 33/18kda. Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.. Target Synonyms: C1-r; complement Component 1; Complement component 1 subcomponent r Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Lead Time

7-11 Business Days

Expression System

Eukaryotic expression

Accession Number

Q8CG16

Gene ID

50909

Expression Region

Met1~Asn707

Theoretical MW

33/18kDa

Conjugate

Unconjugated

Endotoxin Level

<1.0EU/ug, by the LAL method

Isoelectric Point

5.4

Applications

Cell Culture, Activity Assays

Purity

>90% by SDS-PAGE

Format

Freeze-dried powder

Storage

Store at 2-8°C for one month,-80°C for 12 months

Buffer

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Expiration

Stable for 12 months if correctly stored

Reconstitution

Refer to the datasheet/CoA included in the product pouch.

Shipping Condition

Ice packs

Research Area

Signal Transduction, Infection Immunity, Immune Molecule

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 and ISO 13485 certified facilities.

Technical Documents

Selected Citations Featuring Biomatik's Products & Services
Last updated 2020-01-31

 

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SDS-PAGE
The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates. Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B). The full-length (amino acid residues 1-707) of mouse C1r was expressed which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 50 mM Tris, pH 7.5 (Assay Buffer), initiated by addition 50 μL of various concentrations of C1r (diluted by Assay Buffer) to 50 ul substrate mixture of 0.2mM Z-Lys-SBzl•HCl and 0.2 mM DTNB. The final well serves as a negative control with no C1r, replaced with 50 μl assay buffer. Then read in kinetic mode for 5 minutes at an absorbance of 405 nm. The specific activity of recombinant mouse C1r is > 500 pmol/min/ug.

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