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Mouse Thrombopoietin, Active Protein, Cat#RPU60290

Cat#

RPU60290
List Price:
$665.30

 

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Technical Documents:

Target Name

Thrombopoietin

Target Synonyms

THPO; MGDF; MKCSF; ML; MPLLG; MyeloprolifeRative Leukemia Virus Oncogene Ligand; Megakaryocyte Growth And Development Factor; Megakaryocyte Stimulating Factor

Species

Mouse (Mus musculus)

Host

E. coli

Protein Type

Active Protein

Tag

N-Terminal His Tag

Activity

Biologically Active

Applications

Cell Culture, Activity Assays

tid

50ug,665.3;100ug,931.6;1mg,4324.3

Mouse Thrombopoietin, Active Protein is a purified Active Protein. Purity: >90% by SDS-PAGE. Host: E. coli. Endotoxin Level: <1.0EU per 1ug, by the LAL method. Species: Mouse (Mus musculus). Target Name: Thrombopoietin. Accession Number: P40226. Gene ID: 21832. Expression Region: Ser22~Thr356. Tag Info: N-terminal His Tag. Theoretical MW: 40kda. Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.. Target Synonyms: THPO; MGDF; MKCSF; ML; MPLLG; MyeloprolifeRative Leukemia Virus Oncogene Ligand; Megakaryocyte Growth And Development Factor; Megakaryocyte Stimulating Factor Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Lead Time

7-11 Business Days

Expression System

Prokaryotic expression

Accession Number

P40226

Gene ID

21832

Expression Region

Ser22~Thr356

Theoretical MW

40kDa

Conjugate

Unconjugated

Endotoxin Level

<1.0EU/ug, by the LAL method

Isoelectric Point

9.6

Applications

Cell Culture, Activity Assays

Purity

>90% by SDS-PAGE

Format

Freeze-dried powder

Storage

Store at 2-8°C for one month,-80°C for 12 months

Buffer

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Expiration

Stable for 12 months if correctly stored

Reconstitution

Refer to the datasheet/CoA included in the product pouch.

Shipping Condition

Ice packs

Research Area

Hematology

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 and ISO 13485 certified facilities.

Technical Documents

Selected Citations Featuring Biomatik's Products & Services
Last updated 2020-01-31

 

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SDS-PAGE
Thrombopoietin (TPO) is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis.TPO can promote platelet production, aggregation, ECM adhesion, and activation. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Besides, GCSF has been identified as an interactor of TPO, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse TPO and recombinant mouse GCSF. Briefly, biotin-linked TPO were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to GCSF-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30 min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of TPO and GCSF was shown, the EC50 for this effect is 0.68 ug/mL.
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