Trusted by 10,000+ Scientists Since 2002. View Our Customer Testimonials, Selected Publications and 3,000+ Cited Publications at Google Scholar.

Protein Production

custom-protein expression and production service

Recombinant Protein Expression Service

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Biomatik has been proudly serving 10,000+ scientists since 2002. With more than 12 years of extensive experience in custom protein expression, Biomatik proudly offers custom E. coli expression packages with risk-free guarantee. With the risk-free guarantee, there is no cost to you if the project fails. Our overall success rate is over 95% in E. coli expression systems. Please view a selected publication featuring custom recombinant proteins produced by Biomatik:

Please view our top selling Risk-free E. coli Protein Service Packages.
You may also want to check out the 17,000+ Catalog Proteins Biomatik has to offer.

Risk-free E. coli Protein Service PackagesService ContentsKey DeliverablesEDT
Package #PE2001-E-T
Risk-free Guaranteed E. coli Protein Package

from gene synthesis to tagged protein purification

Tag option: His tag, GST tag or Strep tag
Purity: 85% /90% /95%
Quantity: from milligrams to grams
Price: as low as $1890

Optional: Endotoxin removal or Antibody production
Phase I: Gene Synthesis & Cloning
- Codon optimization and Gene synthesis
- Sub-cloning into expression vectors
- Plasmid preparation

Phase II: Protein Expression & Purification
- Pilot expression optimization
- Scale up expression
- Purification (affinity purification)
- QC by SDS-PAGE & WB

- Tagged Protein
(Guaranteed purity & quantity)
- 4ug DNA with the gene insert
- CoA: SDS-PAGE & WB data
6-8 weeks
Package #PE2001-E-TF
Risk-free Guaranteed E. coli Protein Package

from gene synthesis to tag-free protein purification

Tag option: Tag-free (through tag-free production)*
Purity: 85% /90% /95%
Quantity: from milligrams to grams
Price: as low as $2970

Optional: Endotoxin removal
Phase I: Gene Synthesis & Cloning
- Codon optimization and Gene synthesis
- Sub-cloning into expression vectors
- Plasmid preparation

Phase II: Protein Expression & Purification
- Pilot expression optimization
- Scale up expression
- Tag-free purification (SEC and ion exchange)
- QC by SDS-PAGE & MALDI-TOF (optional)

- Tag-free Protein
(Guaranteed purity & quantity)
- 4ug DNA with the gene insert
- CoA: SDS-PAGE & WB data
6-9 weeks
•  Unlike traditional post-production tag removal, our tag-free protein production ensures proper native folding, and no trace of the affinity tag (no tag cleavages).
•  If the expressed protein is insoluble (expressed as inclusion body), refolding will be attempted to make it soluble. Protein purity is determined by by SDS-PAGE, and quantity is determined by Bradford assay. Protein is delivered in freeze powder whenever possible (in neutral buffer, e.g. Tris or PBS, before lyophilization).
•  Biomatik does not accept plasmid supplied by customer unless the plasmid has been proven to give good expression result. Biomatik does not guarantee bioactivity or functionality on custom made proteins. Biomatik does not guarantee 100% success rate in protein expression services. For any protein failed in production due to technical difficulties, customer may or may not be required to pay full or partial price - please refer to the quote for details. Customer shall accept and pay for any proteins which have been successfully produced.

Why Choose Biomatik?

Biomatik has been trusted among top scientists and researchers from around the world for quality products and custom services since 2002. To date, Biomatik has delivered 70,000+ custom made products to our customers worldwide. Learn why Biomatik has been trusted by thousands of researchers worldwide by viewing our customer testimonials, selected publications and 3,000+ publications citing our fine products and services at Google Scholar.

"Our main concern is both of them are hydrophobic with very limited solubility. Biomatik started the protein production process from codon optimization and gene synthesis, the solubility of proteins we got were significantly improved. When we applied these proteins to our ELISA diagnostic assay, they all keep showing highly specific reactivity and very low background. We are very satisfied with the qualities of these proteins. Biomatik run the whole processes very efficiently and we always get our proteins quickly. Their customer support also works well with many helpful professional advices. So we strongly recommend the protein preparation service of Biomatik. Thanks again for your good job." 
Dr. Jia, Centers for Disease Control and Prevention, Atlanta, GA, USA

“We are very satisfied with the polypeptide antibodies you produced for us. They work great for both WB and immunostaining (so far we tested). They are very strong and specific (for WB, I can use at 1:5k-10k dilution). Previously we had bad experience with peptide antibodies from other companies. This is the first time we have nice peptide antibodies. In addition, I like your service and time frame. Also your price is reasonable. I think I will continue use your service in the future.” 
Dr. Hong Han, Dept. of Biology, McGill University, Montreal, Quebec, Canada

   

Phase I: Gene Synthesis

  • Codon optimization for specific expression system
  • Gene Synthesis
  • Sub-cloning into expression vectors
  • Timeline: ~3 weeks

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Phase II: Protein Expression and Purification

  • Pilot expression optimization
  • Scale up expression
  • Purification
  • QC by SDS-PAGE and Western Blot (for tagged proteins)
  • Timeline: 2-4 weeks

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Additional Services (optional)

  • Endotoxin Removal. Timeline: 1-2 weeks
  • Antibody Production. Timeline: 2-3 months for pAb, 5-6 months for mAb. 

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Contact Information

 

Please obtain a quote before ordering, and refer to the quote number when you place an order. Orders are typically confirmed within 24 hours.
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  • Have a Question? Email us
  • Order Online: Order and Payment Form
  • By Fax: 1-877-221-3515 (USA/Canada) or 1-519-231-0140 (International)
  • By Phone: 1-800-836-8089 (USA/Canada) or 1-519-489-7195 (International)

 

 

 

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