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Protein Production

Protein Production

 
 

   Custom Protein Production

 
Biomatik specializes in E.coli protein expression service with risk-free guarantee. With the risk-free guarantee, it is no cost to you if project fails. Our overall success rate is over 95% in E.coli expression systems. Biomatik has been proudly serving 10,000+ scientists since 2002. To date, 52,000+ custom made products have been custom made for our researchers worldwide.

Please view our top selling Guaranteed Protein Packages #PE2001 (E.coli) and Protein Project Sample Report.
You may also check out the catalog antibodies Biomatik has to offer: 15,000+ Catalog Proteins.


Custom Protein Expression Service (E.coli) Highlights

Risk-Free Guarantee: No Protein, No Charge. If project fails, we cover all the costs including gene synthesis cost.
Low Price with Fast Turnaround:  As low as $1575 for 3mg protein at 85%, in as fast as 5 weeks.
Guaranteed Protein Quantity & Purity:  No cost to you if desired protein amount & purity are not produced.
High Success Rate:  Greater than 95% in E. coli expression system.
Total Customization: choose your quantity, purity, His tag, GST tag or tag-free, and endotoxin removal.
One-Stop Services: From codon optimization, gene synthesis & subcloning, protein expression to pAb or mAb production.

   

Phase I: Gene Synthesis

  • Codon optimization for specific expression system
  • Gene Synthesis
  • Sub-cloning into expression vectors
  • Timeline: ~3 weeks

Cartoon Gene Synthesis
   

Phase II: Protein expression and purification (tagged or tag-free* expression)

  • Pilot expression optimization
  • Scale up expression
  • Purification
  • QC by SDS-PAGE and Western Blot (for tagged protein only)
  • Timeline: 2-4 weeks
  • Deliverable: Purified protein at specified requirements and project report
  • * Unlike tag removal, tag-free expression ensures proper native folding and no trace of the tag.

Protein Expression
   

Phase III: Endotoxin Removal (optional)

  • Endotoxin removal for all the glass ware, raw material and equipment
  • Control the contamination during purification process
  • Final elute from endotoxin removal column
  • QC by gel clot assay for endotoxin level
  • Timeline: 1-2 weeks
  • If failed on endotoxin removal, deliver protein and only charge for Phase I + II

Cartoon Endotoxin Removal
   

Customer Testimonials of Our Protein Services

"Our main concern is both of them are hydrophobic with very limited solubility. Biomatik started the protein production process from codon optimization and gene synthesis, the solubility of proteins we got were significantly improved. When we applied these proteins to our ELISA diagnostic assay, they all keep showing highly specific reactivity and very low background. We are very satisfied with the qualities of these proteins. Biomatik run the whole processes very efficiently and we always get our proteins quickly. Their customer support also works well with many helpful professional advices. So we strongly recommend the protein preparation service of Biomatik. Thanks again for your good job."
- Quoted from Dr. Jia
Centers for Disease Control and Prevention
Atlanta, GA, USA 30333




Recent Publications Citing Biomatik's Custom Protein and Antibody Production

Baerlecken, N.T., Linnemann, A., Gross, W.L., Moosig, F., Vazquez-Rodriguez, T.R., Gonzalez-Gay, M.A., Martin, J., Kötter, I., Henes, J.C., Melchers, I., Vaith, P., Schmidt, R.E. and Witte, T. (2012). Association of ferritin autoantibodies with giant cell arteritis/polymyalgia rheumatic. Ann. Rheum. Dis. 71, 943-947.

Bedford, A., Chen, T., Huynh, E., Zhu, C., Medeiros, S., Wey, D., de Lange, C. and Li, J. (2015). Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved. J. Biotechnol. 196-197, 9-19.

Kazemi-Lomedasht, F., Behdani, M., Bagheri, K.P., Anbouhi, M.H., Abolhassani, M., Khanahmad, H., Shahbazzadeh, D. and Mirzahoseini, H. (2014). Expression and purification of functional human vascular endothelial growth factor-A121; The most important angiogenesis factor.Adv. Pharm. Bull. 4(4), 323-328.

Liu, Y., Zheming, W., Liu, J., Levar, C., Edwards, M.J., Babauta, J.T., Kennedy, D.W., Shi, Z., Beyenal, H., Bond, D.R., Clarke, T.A., Butt, J.N., Richardson, D.J., Rosso, K.M., Zachara, J.M., Fredrickson, J.K. and Shi, L. (2014). A trans-outer membrane porin-cytochrome protein complex for extracellular electron transfer by Geobacter sulfurreducens PCA. Environ. Microbiol. Rep. 6(6), 776-785.

Mathers, C., Spencer, C.M. and Munger, J. (2014). Distinct domains within the human cytomegalovirus UL26 protein are important for wildtype viral replication and virion stability. PLoS ONE 9(12), e114927.

Moon, Y.M., Metoxen, A.J., Lerning, M.T., Whaley, M.A. and O’Tousa, J.E. (2014). Rhodopsin management during the light–dark cycle of Anopheles gambiae mosquitoes. J. Insect Physiol. 70, 88-93.

Reynolds, C.J., Jones, C., Blohmke, C.J., Darton, T.C., Goudet, A., Sergeant, R., Maillere, B., Pollard, A.J., Altmann, D.M. and Boyton, R.J. (2014). The serodominant secreted effector protein of Salmonella, SseB, is a strong CD4 antigen containing an immunodominant epitope presented by diverse HLA class II alleles. Immunology 143(3), 438-446.

Shi, L., Ko, S., Ko, M.L., Kim, A.J. and Ko, G.Y-P. (2015). Peptide Lv augments L-type voltage-gated calcium channels through vascular endothelial growth factor receptor 2 (VEGFR2) signaling. BBA-Mol. Cell Res. 1853(5), 1154-1164.

Wadwa, J., Chu, Y-H., Nguyen, N., Henson, T., Figueroa, A., Llanos, R., Ackland, M.L., Michalczyk, A., Fullriede, H., Brennan, G., Mercer, J.F.B. and Linder, M.C. (2014). Effects of ATP7A overexpression in mice on copper transport and metabolism in lactation and gestation. Physl. Rep. 2, e00195.

Last updated 2015-03-23

   

Sample Protein Project Report 1/1

 


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    Why Choose Biomatik?

Biomatik has been serving world wide researchers since 2002. Our team has extensive experience in E. coli protein expression and purification service. Researchers come to Biomatik not only for our competitive prices, fast turnaround time, but also for our quality, consistency and reputation.
      View Biomatik's Cited Publications at Google Scholar: Click Here

      "Our main concern is both of them are hydrophobic with very limited solubility. Biomatik started the protein production process from codon optimization and gene synthesis, the solubility of proteins we got were significantly improved. When we applied these proteins to our ELISA diagnostic assay, they all keep showing highly specific reactivity and very low background. We are very satisfied with the qualities of these proteins. Biomatik run the whole processes very efficiently and we always get our proteins quickly. Their customer support also works well with many helpful professional advices. So we strongly recommend the protein preparation service of Biomatik. Thanks again for your good job."
      - Dr. Jia, Centers for Disease Control and Prevention, Atlanta, GA, USA

   Protein Expression Service Knowledge Base


 

   Ordering Information

 
  
Please obtain an official quote before ordering: Request Quote. Biomatik accepts online, phone, fax and email orders. Please refer to the Quote/Reference number when you place an order - orders are typically confirmed within 24 hours.
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    • By Fax: 1-877-221-3515 (USA/Canada) or 1-519-231-0140 (International).
    • Phone Us: 1-800-836-8089 (USA/Canada) or 1-519-489-7195 (International).

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