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Cat#

CAC12004
List Price:
$252.00

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Target Name

ACLY

Target Synonyms

ATP-citrate synthase (EC 2.3.3.8) (ATP-citrate (pro-S-)-lyase) (ACL) (Citrate cleavage enzyme); ACLY

Species

Human (Homo sapiens)

Species Reactivity

Human (Homo sapiens)

Antibody Type

Recombinant Antibody

Host

HEK293F Cell

Isotype

Rabbit IgG

Clonality

Monoclonal

Immunogen

A Synthesized Peptide Derived From Human Atp Citrate Lyase

Applications

ELISA, WB, IF, FC

Acly Recombinant Antibody is a Human (Homo sapiens) Recombinant Antibody to ACLY. The Acly Recombinant Antibody has been validated for the following applications: ELISA, WB, IF, FC. Host: HEK293F Cell. Species: Human (Homo sapiens). Immunogen: A Synthesized Peptide Derived From Human Atp Citrate Lyase. Applications: ELISA, WB, IF, FC. Target Synonyms: ACLY. Accession Number: P53396. Storage: 2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles. Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Acly Recombinant Antibody is a Human (Homo sapiens) Recombinant Antibody to ACLY. The Acly Recombinant Antibody has been validated for the following applications: ELISA, WB, IF, FC.

Lead Time

7-11 Business Days

Accession Number

P53396

Clone Number

3A5

Conjugate

Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.

Applications

ELISA, WB, IF, FC

Purification

Affinity-Chromatography

Format

Liquid

Storage

2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.

Buffer

Rabbit IgG in Phosphate Buffered Saline, pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol

Expiration

12 months

Shipping Condition

Ice packs

Research Area

Cancer, Metabolism, Signal Transduction

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 certified facilities.

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Western Blot; Positive WB detected in: L02 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, A549 whole cell lysate; All lanes: ACLY antibody at 1:1500; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 121, 120, 92 kDa; Observed band size: 120 kDa
Immunofluorescence staining of HepG2 Cells with CAC12004 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay histogram showing Hela cells stained with CAC12004 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106 cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.

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