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Cat#

CAC10049
List Price:
$252.00
Ultra Sensitive IHC 

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Target Name

MTOR

Target Synonyms

Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; R

Species

Human (Homo sapiens)

Species Reactivity

Human (Homo sapiens)

Antibody Type

Recombinant Antibody

Host

HEK293F Cell

Isotype

Rabbit IgG

Clonality

Monoclonal

Immunogen

A Synthesized Peptide Derived From Human Phospho-Mtor (S2448)

IHC Validation

IHC Validated

Applications

ELISA, WB, IHC, IF

Phospho-Mtor (S2448) Recombinant Antibody is a Human (Homo sapiens) Recombinant Antibody to MTOR. The Phospho-Mtor (S2448) Recombinant Antibody has been validated for the following applications: ELISA, WB, IHC, IF. Host: HEK293F Cell. Species: Human (Homo sapiens). Immunogen: A Synthesized Peptide Derived From Human Phospho-Mtor (S2448). Applications: ELISA, WB, IHC, IF. Target Synonyms: MTOR. Accession Number: P42345. Storage: 2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles. Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Phospho-Mtor (S2448) Recombinant Antibody is a Human (Homo sapiens) Recombinant Antibody to MTOR. The Phospho-Mtor (S2448) Recombinant Antibody has been validated for the following applications: ELISA, WB, IHC, IF.

Lead Time

7-11 Business Days

Accession Number

P42345

Clone Number

1G6

Conjugate

Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.

Applications

ELISA, WB, IHC, IF

Purification

Affinity-Chromatography

Format

Liquid

Storage

2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.

Buffer

Rabbit IgG in Phosphate Buffered Saline, pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol

Expiration

12 months

Shipping Condition

Ice packs

Research Area

Cell Biology

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 certified facilities.

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Western Blot; Positive WB detected in Hela whole cell lysate,A549 whole cell lysate; All lanes Phospho-MTOR antibody at 0.825µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 289 KDa; Observed band size: 289 KDa;
IHC image diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC10049 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

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