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Cat#

CAC11123
List Price:
$199.20
Ultra Sensitive IHC  Best Seller 

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Target Name

ACAA2

Target Synonyms

3 ketoacyl CoA thiolase; 3 ketoacyl CoA thiolase mitochondrial; 3-ketoacyl-CoA thiolase; mitochondrial; 3-oxoacyl-CoA thiolase; mitochondrial; ACAA 2; Acaa2; Acetyl CoA acyltransferase 2; Acetyl CoA acyltransferase; Acetyl Coenzyme A acyltransferase

Species

Human (Homo sapiens)

Species Reactivity

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Antibody Type

Polyclonal Antibody

Host

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

Recombinant Human 3-Ketoacyl-Coa Thiolase, Mitochondrial Protein (188-257Aa)

IHC Validation

IHC Validated

Applications

ELISA, WB, IHC, IF, IP

Acaa2 Polyclonal Antibody is a Human (Homo sapiens) Polyclonal Antibody to ACAA2. The Acaa2 Polyclonal Antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP. Host: Rabbit. Species: Human (Homo sapiens). Immunogen: Recombinant Human 3-Ketoacyl-Coa Thiolase, Mitochondrial Protein (188-257Aa). Applications: ELISA, WB, IHC, IF, IP. Target Synonyms: ACAA2. Accession Number: P42765. Storage: 2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles. Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Acaa2 Polyclonal Antibody is a Human (Homo sapiens) Polyclonal Antibody to ACAA2. The Acaa2 Polyclonal Antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP.

Lead Time

7-11 Business Days

Accession Number

P42765

Conjugate

Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.

Applications

ELISA, WB, IHC, IF, IP

Purification

>95%, Protein G Purified

Format

Liquid

Storage

2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.

Buffer

Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Expiration

12 months

Shipping Condition

Ice packs

Research Area

Tags And Cell Markers

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 certified facilities.

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Western Blot; Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue; All lanes: ACAA2 antibody at 2.4µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 42 kDa; Observed band size: 42 kDa
IHC image diluted at 1:700 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescent analysis of HepG2 cells using CAC11123 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating ACAA2 in A549 whole cell lysate ; , Lane 1: Rabbit control IgG (1ug) instead in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11123 (6ug) + A549 whole cell lysate (500ug); , Lane 3: A549 whole cell lysate (10ug)

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