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Cat#

CAC07011
List Price:
$199.20
Ultra Sensitive IHC 

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Target Name

ACTB

Target Synonyms

A26C1A; A26C1B; ACTB; ACTB_HUMAN; Actin beta; Actin cytoplasmic 1; Actin; cytoplasmic 1; N-terminally processed; Actx; b actin; b-actin; Beta cytoskeletal actin; Beta-actin; BRWS1; E430023M04Rik; Melanoma X actin; MGC128179; PS1TP5 binding protein 1;

Species

Human (Homo sapiens)

Species Reactivity

Human (Homo sapiens)

Antibody Type

Polyclonal Antibody

Host

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

Recombinant Human Actin, Cytoplasmic 1 Protein (102-375Aa)

IHC Validation

IHC Validated

Applications

ELISA, WB, IHC, IF

Actb Polyclonal Antibody is a Human (Homo sapiens) Polyclonal Antibody to ACTB. The Actb Polyclonal Antibody has been validated for the following applications: ELISA, WB, IHC, IF. Host: Rabbit. Species: Human (Homo sapiens). Immunogen: Recombinant Human Actin, Cytoplasmic 1 Protein (102-375Aa). Applications: ELISA, WB, IHC, IF. Target Synonyms: ACTB. Accession Number: P60709. Storage: 2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles. Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Actb Polyclonal Antibody is a Human (Homo sapiens) Polyclonal Antibody to ACTB. The Actb Polyclonal Antibody has been validated for the following applications: ELISA, WB, IHC, IF.

Lead Time

7-11 Business Days

Accession Number

P60709

Conjugate

Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.

Applications

ELISA, WB, IHC, IF

Purification

>95%, Protein G Purified

Format

Liquid

Storage

2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.

Buffer

Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Expiration

12 months

Shipping Condition

Ice packs

Research Area

Isotype/Loading Controls

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 certified facilities.

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Western Blot; Positive WB detected in: HepG2 whole cell lysate; All lanes: ACTB antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 42 kDa; Observed band size: 42 kDa
Immunohistochemistry of paraffin-embedded human endometrial cancer using CAC07011 at dilution of 1:100
IHC image diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:500 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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