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Cat#

CAC07118
List Price:
$199.20
Ultra Sensitive IHC 

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Target Name

AQP4

Target Synonyms

AQP 4; AQP-4; AQP4; AQP4_HUMAN; Aquaporin type 4; Aquaporin-4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; Mercurial-insensitive water channel; MGC22454; MIWC; WCH 4; WCH4

Species

Human (Homo sapiens)

Species Reactivity

Human (Homo sapiens), Mouse (Mus musculus)

Antibody Type

Polyclonal Antibody

Host

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

Recombinant Human Aquaporin-4 Protein (253-323Aa)

IHC Validation

IHC Validated

Applications

ELISA, WB, IHC, IF

Aqp4 Polyclonal Antibody is a Human (Homo sapiens) Polyclonal Antibody to AQP4. The Aqp4 Polyclonal Antibody has been validated for the following applications: ELISA, WB, IHC, IF. Host: Rabbit. Species: Human (Homo sapiens). Immunogen: Recombinant Human Aquaporin-4 Protein (253-323Aa). Applications: ELISA, WB, IHC, IF. Target Synonyms: AQP4. Accession Number: P55087. Storage: 2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles. Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Aqp4 Polyclonal Antibody is a Human (Homo sapiens) Polyclonal Antibody to AQP4. The Aqp4 Polyclonal Antibody has been validated for the following applications: ELISA, WB, IHC, IF.

Lead Time

7-11 Business Days

Accession Number

P55087

Conjugate

Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.

Applications

ELISA, WB, IHC, IF

Purification

>95%, Protein G Purified

Format

Liquid

Storage

2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.

Buffer

Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Expiration

12 months

Shipping Condition

Ice packs

Research Area

Signal Transduction

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 certified facilities.

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Western blot; All lanes: AQP4 antibody at 2µg/ml; Lane 1: Mouse heart tissue; Lane 2: Mouse brain tissue; Lane 3: Mouse kidney tissue; Secondary; Goat polyclonal to rabbit IgG at 1/10000 dilution; Predicted band size: 35, 33 kDa; Observed band size: 35 kDa
IHC image diluted at 1:370 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:370 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of U251 cells with CAC07118 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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