Tissue homogenates and other biological fluids.
Estimated Turnaround Time
7-11 business days
SPTLC2 / Serine Palmitoyltransferase, Long Chain Base Subunit 2
LCB2; SPT2; Long chain base biosynthesis protein 2; Serine-palmitoyl-CoA transferase 2
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Short term: 4°C; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
This assay has high sensitivity and excellent specificity for detection of Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2). No significant cross-reactivity or interference between Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) and analogues was observed.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Inhibitory Activity Protein 1 (MIA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanoma Inhibitory Activity Protein 1 (MIA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanoma Inhibitory Activity Protein 1 (MIA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanoma Inhibitory Activity Protein 1 (MIA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay procedure summary
1. Prepare all reagents, samples and standards; 2. Add 100µl standard or sample to each well. Incubate 2 hours at 37°C ; 3. Aspirate and add 100µl prepared Detection Reagent A. Incubate 1 hour at 37°C ; 4. Aspirate and wash 3 times; 5. Add 100µl prepared Detection Reagent B. Incubate 30 minutes at 37°C ; 6. Aspirate and wash 5 times; 7. Add 90µl Substrate Solution. Incubate 10-20 minutes at 37°C ; 8. Add 50µl Stop Solution. Read at 450nm immediately.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
For Research Use Only. Not for use in diagnostic procedures. Not for human or animal drug or food use.
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in the kit before experiment.
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