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Human Caspase 3 (CASP3), Active Protein, Cat#RPU56326

Cat#

RPU56326
List Price:
$653.40

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Target Name

Caspase 3 (CASP3)

Target Synonyms

CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1

Species

Human (Homo sapiens)

Host

E. coli

Protein Type

Prokaryotic Protein

Tag

N-terminal His Tag

Activity

Biologically Active

Applications

Cell Culture, Activity Assays

Human Caspase 3 (CASP3), Active Protein is a purified Prokaryotic Protein. Purity: >90% by SDS-PAGE. Host: E. coli. Endotoxin Level: <1.0EU per 1ug, by the LAL method. Species: Human (Homo sapiens). Target Name: Caspase 3 (CASP3). Target Synonyms: CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1. Accession Number: p42574. Gene ID: 836. Expression Region: Ser29~His277. Tag Info: N-terminal His Tag. Theoretical MW: 14/18/20kDa. Buffer: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose. Storage: -20°C. Avoid repeated freeze/thaw cycles. Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Human Caspase 3 (CASP3), Active Protein is a purified Prokaryotic Protein.

Lead Time

7-11 Business Days

Expression System

Prokaryotic Expression

Accession Number

p42574

Gene ID

836

Expression Region

Ser29~His277

Theoretical MW

14/18/20kDa

Conjugate

Unconjugated

Endotoxin Level

<1.0EU per 1ug, by the LAL method

Isoelectric Point

7.1

Applications

Cell Culture, Activity Assays

Purity

>90% by SDS-PAGE

Format

Freeze-Dried Powder

Storage

-20°C. Avoid repeated freeze/thaw cycles.

Buffer

PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Expiration

Stable for 12 months if correctly stored

Reconstitution

Refer to the datasheet/CoA included in the product pouch.

Shipping Condition

Ice packs

Research Area

Signal Transduction

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 and ISO 13485 certified facilities.

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Figure . SDS-PAGE
Caspase 3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6 and 7; and the protein itself is processed and activated by caspases 8, 9, and 10. Caspase 3 can hydrolyze the peptide substrate acetyl-Asp-Glu-Val-Asp-p-nitroanilide (Ac-DEVD-pNA) resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405nm. Thus the activity of recombinant human caspase 3 can be measured by calculate the concentration of the pNA released from the substrate. The reaction was performed in adding 50μL 2×buffer (50mM HEPES, 100mM NaCl, 10mM DTT, 2mM EDTA, 10% glycerol) to 96 well plates, then add 50μL various concentration of caspe 3 (diluted by 1×buffer, 25mM HEPES, 50mM NaCl, 5mM DTT, 1mM EDTA, 5% glycerol) to each well, finally, add 5μL 4mmol Ac-DEVD-pNA to each well. Cover the 96 well plates and incubate at 37℃ for 2h. p-Nitroaniline (pNA) standard curve prepare by double dilute 200μM pNA with 1×buffer and record the OD value at 405nm. Calculate the caspase 3 activity in pmol of pNA released per min per μg recombinat human caspase 3.The specific activity of recombinant human caspase 3 is 2196pmol/min/μg.
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