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Human Tubulin Beta (TUBb), Active Protein, Cat#RPU54943

Cat#

RPU54943
List Price:
$819.50

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Target Name

Tubulin Beta (TUBb)

Target Synonyms

TUB-B; TUBB5; TUBB1; M40

Species

Human (Homo sapiens)

Host

E. coli

Protein Type

Prokaryotic Protein

Tag

N-terminal His Tag

Activity

Biologically Active

Applications

Cell Culture, Activity Assays

Human Tubulin Beta (TUBb), Active Protein is a purified Prokaryotic Protein. Purity: >95% by SDS-PAGE. Host: E. coli. Endotoxin Level: <1.0EU per 1ug, by the LAL method. Species: Human (Homo sapiens). Target Name: Tubulin Beta (TUBb). Target Synonyms: TUB-B; TUBB5; TUBB1; M40. Accession Number: P07437. Gene ID: 203068. Expression Region: Val170~Val419. Tag Info: N-terminal His Tag. Theoretical MW: 32kDa. Buffer: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose. Storage: -20°C. Avoid repeated freeze/thaw cycles. Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Human Tubulin Beta (TUBb), Active Protein is a purified Prokaryotic Protein.

Lead Time

7-11 Business Days

Expression System

Prokaryotic Expression

Accession Number

P07437

Gene ID

203068

Expression Region

Val170~Val419

Theoretical MW

32kDa

Conjugate

Unconjugated

Endotoxin Level

<1.0EU per 1ug, by the LAL method

Isoelectric Point

6.6

Applications

Cell Culture, Activity Assays

Purity

>95% by SDS-PAGE

Format

Freeze-Dried Powder

Storage

-20°C. Avoid repeated freeze/thaw cycles.

Buffer

PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Expiration

Stable for 12 months if correctly stored

Reconstitution

Refer to the datasheet/CoA included in the product pouch.

Shipping Condition

Ice packs

Research Area

Neuro Science

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 and ISO 13485 certified facilities.

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Tubulin Beta (TUBb) in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. It participate in many essential cellular processes, including mitosis. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Both α and β tubulins have a mass of around 50kDa and are thus in a similar range compared to actin with ~42kDa. TUBb is one of six members of the tubulin superfamily, which binding drugs to kill cancerous cells by inhibiting microtubule dynamics. Besides, Myxovirus Resistance 1 (MX1) has been identified as an interactor of TUBb, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TUBb and recombinant human MX1. Briefly, TUBb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MX1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TUBb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TUBb and MX1 was shown in Figure 1, and this effect was in a dose dependent manner.
Human Tubulin Beta (TUBb), Active Protein, Cat#RPU54943
Figure 3. SDS-PAGE
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